Mouse anti-Human Lambda Light Chain (unconjugated), Clone HP6054, IgG2a, Purified

Concentrated Antibody for Immunohistochemistry

SKU:
K037
Kategorie
Quantity
0, 1 ml

Background

Monoclonal Anti-Human Lambda Light Chains is specific for the lambda light chains of human immunoglobulins (all isotypes), and is non-reactive with kappa light chains. The antibody recognizes both the heavy chain-bound and the free (Bence Jones) human lambda light chain in an ELISA. The estimated association constant of this antibody to its ligand is 1.2 x 109 L/M.
Immunoglobulins are composed of two heavy and two light polypeptide chains held together by noncovalent forces and usually by interchain disulfide bridges. The various types of human (and other mammalian) immunoglobulins contain one of the two existing light chain types, either kappa or lambda in which multiple structural differences are reflected in antigenic variety mainly the N-terminal (variable) domain of the chains. In monoclonal disorders, such as myeloma and macroglobulinemia, an increase in the level of a single immunoglobulin class can be accompanied by disproportionate increases in either lambda or kappa light chains. In many cases of B cell malignancy there is an increased production of light chains which are not combined with heavy chains. These circulate in the various body fluids (blood, cerebral spinal fluid, and tissues) and are found in enormous amounts as free molecules in the urine
(Bence Jones paraproteins).

Volume/Quantity

1 ml

Product Form

Ascites fluid – liquid

Approx. Protein Concentrations

IgG concentration 7.3 mg/ml

Preservatives Stabilisers

0.1% Sodium Azide (NaN3)

Immunogen

Purified human IgG molecules in the urine (Bence Jones paraproteins). Myeloma proteins covalently coupled to polyaminostyrene (PAS) microbeads were used as the Uses immunogen.

Clone Number

HP-6054

Isotype

IgG2a (Mouse)

Suggested Working Dilution

FlowCytometry Not tested
Immunohistology-frozen Yes 1/500
Immunohistology-paraffin Yes 1/500
Immunohistology-resin Not tested
ELISA Yes 1/1000
Immunoprecipitation Yes
Western Blotting Yes 1/1000
Radioimmunoassay Yes

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Westernblotting

Chemiluminescent Substrate

DuoLuX Chemiluminescent Substrate for Alkaline Phosphatase (AP) (LINARIS CatNo ESA6605) and Peroxidase (POD) (LINARIS CatNo ESP6604).

Western Blotting Immunodetection Kit

ABC-AmP Chromogenic Detection Kit anti-Mouse IgG (LINARIS CatNo EDA6402) or anti-Rabbit IgG
(LINARIS CatNo EDA6401)

Weight Standard

Biotinylated protein molecular weight marker (LINARIS CatNo RPB1400). Molecular weight range from
19kD to 222kD.

Immunohistology

Pre-treatment

Proteolytic digestion prior to staining of paraffin sections with Pronase (LINARIS CatNo. E110) increase the staining sensitivity.

Recommended Secondary Reagents

F(ab‘)2 rabbit anti-mouse IgG HRP conjugate – (LINARIS CatNo LST0013B)
ABC-Kit Mouse IgG-POD Labelling (LINARIS CatNo EDP4002)
DAB-Substrate for POD (LINARIS CatNo E108)
ABC-Kit Mouse IgG AP Labelling (LINARIS CatNo EDA5002)
BCIP/NBT Substrate for AP (LINARIS CatNo ESA5400)

Recommended Negative Controls

Mouse IgG2a Negative Control (LINARIS CatNo ITC0929)

Storage Conditions

Store at 2-8°C for one month or at –20°C for longer!
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a
precipitate we recommend microcentrifugation before use.

Shelf Life

12 months from date of despatch.

Reimer, C.B., et al., Hybridoma 3, 263 (1984)

Specificity

Lambda Light Chain

Species Reactivity

Human

Host / Source

Mouse

Isotype

IgG2a

Application

IHC-F, IHC-P

Clone

HP6054

Antigen

Human Lambda Light Chain

Quantity

0, 1 ml

Format

Purified

Storage Temperature

2-8 °C

Shipping Temperature

2-8 °C

Dokumente

pdf Datasheet