Mouse anti-Human Lambda Light Chain (unconjugated), Clone HP6054, IgG2a, Purified
Concentrated Antibody for Immunohistochemistry
Background
Monoclonal Anti-Human Lambda Light Chains is specific for the lambda light chains of human immunoglobulins (all isotypes), and is non-reactive with kappa light chains. The antibody recognizes both the heavy chain-bound and the free (Bence Jones) human lambda light chain in an ELISA. The estimated association constant of this antibody to its ligand is 1.2 x 109 L/M.
Immunoglobulins are composed of two heavy and two light polypeptide chains held together by noncovalent forces and usually by interchain disulfide bridges. The various types of human (and other mammalian) immunoglobulins contain one of the two existing light chain types, either kappa or lambda in which multiple structural differences are reflected in antigenic variety mainly the N-terminal (variable) domain of the chains. In monoclonal disorders, such as myeloma and macroglobulinemia, an increase in the level of a single immunoglobulin class can be accompanied by disproportionate increases in either lambda or kappa light chains. In many cases of B cell malignancy there is an increased production of light chains which are not combined with heavy chains. These circulate in the various body fluids (blood, cerebral spinal fluid, and tissues) and are found in enormous amounts as free molecules in the urine
(Bence Jones paraproteins).
| Specificity | Lambda Light Chain |
|---|---|
| Species Reactivity | Human |
| Host / Source | Mouse |
| Isotype | IgG2a |
| Application | IHC-F, IHC-P |
| Clone | HP6054 |
| Antigen | Human Lambda Light Chain |
| Quantity | 0, 1 ml |
| Format | Purified |
| Storage Temperature | 2-8 °C |
| Shipping Temperature | 2-8 °C |
Datasheet