Mouse anti-Human Melanoma (unconjugated), Clone HMB45, IgG1, Purified

Concentrated Antibody for Immunohistochemistry

SKU:
K050
Category
Quantity
0, 5 ml
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Background

Melanocytes are pigmented, melanin containing cells being located mainly in the epidermis. They protect tissues from UVB irradiation of the sun. The antibody HMB45 reacts with fetal and neonatal Melanocytes, with junctional and blue naevus cells, but not with intradermal naevi and adult
melanocytes.

Volume/Quantity

0.2 ml/100 tests

Product Form

Tissue Culture Supernatant – liquid

Buffer

0.05 mol/L Tris-HCl, pH 7.2

Preservatives Stabilisers

15 mM Sodium Azide (NaN3)

Immunogen

Extract of pigmented melanoma metastases from lymphnodes

Clone Number

HMB-45

Isotype

IgG1 (Mouse)

Specificity

The antibody HMB45 is used for the discrimination of Melanoma from normal Melanocytes. Melanoic Schwannoma Clear Cell Sarcoma will also be stained. Negative are tumours of epithelial, lymphoid,
glial and mesenchymal origin.

Suggested Working Dilution

FlowCytometry Not tested
Immunohistology-frozen Yes
Immunohistology-paraffin Yes 1/50
Immunohistology-resin Not tested
ELISA Not tested
Immunoprecipitation Not tested
Western Blotting Not tested
Radioimmunoassay Not tested

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Immunohistology

Positive Control Tissue

Melanoma

Recommended Secondary Reagents

F(ab’)2 rabbit anti-mouse IgG HRP conjugate – (LINARIS CatNo LST0013B)
ABC-Kit Mouse IgG-POD Labelling (LINARIS CatNo EDP4002)
DAB-Substrate for POD (LINARIS CatNo E108)
ABC-Kit Mouse IgG AP Labelling (LINARIS CatNo EDA5002)
BCIP/NBT Substrate for AP (LINARIS CatNo ESA5400)

Recommended Negative Controls

Mouse IgG1 Negative Control (LINARIS CatNo ITC0928)

Storage Conditions

Store at 2-8°C!
Should this product contain a precipitate we recommend microcentrifugation before use.

Shelf Life

12 months from date of despatch.

Gown AM and Vogel AM. Monoclonal antibodies to intermediate filament proteins of human cells: unique and cross-reacting antibodies. J Cell Biol 1982; 95:414

Gown AM and Vogel AM. Monoclonal antibodies to human intermediate filament proteins. III. Analysis of tumors. Amer J Clin Pathol 1985; 84:413

Moll R, et al. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors, and cultured cells. Cell 1982; 31:11

Dairkee SH, et al. Expression of basal and luminal epithelium-specific keratins in normal benign and malignant breast tissue. J Natl Canc Inst 1988; 80:691

O’Malley FP, et al. Usefulness of immunoperoxidase staining with high-molecular-weight cytokeratin in the differential diagnosis of small-acinar lesions of the prostate gland. Virch Arch A Pathol Anat 1990; 417:191

Gown AM and Vogel AM. Monoclonal antibodies to intermediate filament proteins of human cells. II. Distribution of filament proteins in normal human tissue. Amer J Pathol 1984; 114:309

Specificity

Melanoma
Species Reactivity

Human
Host / Source

Mouse
Isotype

IgG1
Application

IHC-F, IHC-P
Clone

HMB45
Antigen

Human Melanoma
Quantity

0, 5 ml
Format

Purified
Storage Temperature

2-8 °C
Shipping Temperature

2-8 °C

Documents

pdf Datasheet